Tg Abs (normal range, <100> immunoradiometric assay kit (Biochem Immunosystem, Bologna, Italy) whereas TPO Abs (normal range, <10> by RIA kit set (DiaSorin, Inc., Saluggia, Italy). The detection limit of the assays, the intra- and interassay coefficients of variation were: 5.0 U/ml, 3.9% and 6.9%, for Tg Ab and 0.7 U/ml, 2.5% and 6.6%, for TPO Abs, respectively. Quality control pools at low, normal, and high concentrations for all parameters were present in each assay. Samples were assayed in duplicate. Serum IP-10/CXCL10 levels were assayed by a quantitative sandwich immunoassay using a commercially available kit (R&D Systems, Minneapolis, MN), with a sensitivity ranging from 0.41 and 4.46 pg/ml and a mean minimum detectable dose of 1.67 pg/ml. The intra- and interassay coefficients of variation were 3% and 6.9%.
http://ajp.amjpathol.org/cgi/content/full/161/1/195
Rabu, 13 Agustus 2008
IP-10/CXCL10 and Mig/CXCL9 expression
IP-10/CXCL10 and Mig/CXCL9 expression in thyroid tissue specimens from patients with GD. A: Section of normal thyroid hybridized with anti-sense IP-10/CXCL10 mRNA probe showing virtually no signal. B: Section of a TA hybridized with anti-sense IP-10/CXCL10 mRNA probe showing virtually no signal. C: High signal in the thyroid specimen from a patient with GD hybridized with anti-sense IP-10/CXCL10 mRNA probe. D: Absence of signal in a consecutive section hybridized with a sense IP-10/CXCL10 mRNA probe. E: High-power magnification of the thyroid follicular epithelium from the same patient with GD hybridized with anti-sense IP-10/CXCL10 mRNA probe. F: Section of normal thyroid hybridized with anti-sense Mig/CXCL9 mRNA probe showing virtually no signal. G: Section of a TA hybridized with anti-sense Mig/CXCL9 mRNA probe showing virtually no signal. H: High signal in the thyroid specimen from a patient with GD hybridized with anti-sense Mig/CXCL9 mRNA probe. I: High-power magnification of the thyroid follicular epithelium from the same patient with GD hybridized with anti-sense Mig/CXCL9 mRNA probe. Dark-field original magnifications: x100 (A–D, F–H); x1000 (E, I).
http://ajp.amjpathol.org/cgi/content/full/161/1/195